Construct: shRNA TRCN0000285392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015239.2-3463s21c1
DNA Barcode:
TATACCATGGAGAGTACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGTPBP1 (23287)
Gene Description:
ATP/GTP binding protein 1
Transcript:
RefSeq NM_015239.2 (CURRENT)
Match location:
Position 3463 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATACCATGGAGAGTACTTTA
Hairpin Sequence:
5'-CCGG-TATACCATGGAGAGTACTTTA-CTCGAG-TAAAGTACTCTCCATGGTATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATACCATGGAGAGTACTTTACTCGAGTAAAGTACTCTCCATGGTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATACCATGGAGAGTACTTTACTCGAGTAAAGTACTCTCCATGGTATA-3'

Other clones with same target sequence:

(none)