Construct: shRNA TRCN0000285406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003893.4-913s21c1
DNA Barcode:
GCTACTTCCGCAGCATCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LDB1 (8861)
Gene Description:
LIM domain binding 1
Transcript:
RefSeq NM_003893.4 (NON-CURRENT)
Match location:
Position 913 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTTCCGCAGCATCTTTG
Hairpin Sequence:
5'-CCGG-GCTACTTCCGCAGCATCTTTG-CTCGAG-CAAAGATGCTGCGGAAGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTTCCGCAGCATCTTTGCTCGAGCAAAGATGCTGCGGAAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTTCCGCAGCATCTTTGCTCGAGCAAAGATGCTGCGGAAGTAGC-3'

Other clones with same target sequence:

(none)