Construct: shRNA TRCN0000285499
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_022104.3-1033s21c1
- DNA Barcode:
- GCAAGGTGGTAGACAAAGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PCIF1 (63935)
- Gene Description:
- PDX1 C-terminal inhibiting factor 1
- Transcript:
- RefSeq NM_022104.3 (NON-CURRENT)
- Match location:
- Position 1033 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000285499)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAAGGTGGTAGACAAAGGAT
- Hairpin Sequence:
-
5'-CCGG-GCAAGGTGGTAGACAAAGGAT-CTCGAG-ATCCTTTGTCTACCACCTTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAAGGTGGTAGACAAAGGATCTCGAGATCCTTTGTCTACCACCTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAAGGTGGTAGACAAAGGATCTCGAGATCCTTTGTCTACCACCTTGC-3'
Other clones with same target sequence:
-
TRCN0000156865
