Construct: shRNA TRCN0000285546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005119.3-2377s21c1
DNA Barcode:
GCCTTGATATTGAACGTCGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THRAP3 (9967)
Gene Description:
thyroid hormone receptor associated protein 3
Transcript:
RefSeq NM_005119.3 (NON-CURRENT)
Match location:
Position 2377 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGATATTGAACGTCGTA
Hairpin Sequence:
5'-CCGG-GCCTTGATATTGAACGTCGTA-CTCGAG-TACGACGTTCAATATCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGATATTGAACGTCGTACTCGAGTACGACGTTCAATATCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGATATTGAACGTCGTACTCGAGTACGACGTTCAATATCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000022112