Construct: shRNA TRCN0000285618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026662.4-335s21c1
DNA Barcode:
CGTGCAAGATTGCGTCCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prps2 (110639)
Gene Description:
phosphoribosyl pyrophosphate synthetase 2
Transcript:
RefSeq NM_026662.4 (NON-CURRENT)
Match location:
Position 335 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCAAGATTGCGTCCTCAT
Hairpin Sequence:
5'-CCGG-CGTGCAAGATTGCGTCCTCAT-CTCGAG-ATGAGGACGCAATCTTGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCAAGATTGCGTCCTCATCTCGAGATGAGGACGCAATCTTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCAAGATTGCGTCCTCATCTCGAGATGAGGACGCAATCTTGCACG-3'

Other clones with same target sequence:

  1. TRCN0000025540