Construct: shRNA TRCN0000285723
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021880.2-1750s21c1
- DNA Barcode:
- GCCTGTAATTAAGCTAGTTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Prkar1a (19084)
- Gene Description:
- protein kinase, cAMP dependent regulatory, type I, alpha
- Transcript:
- RefSeq NM_021880.2 (NON-CURRENT)
- Match location:
- Position 1750 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000285723)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTGTAATTAAGCTAGTTTA
- Hairpin Sequence:
-
5'-CCGG-GCCTGTAATTAAGCTAGTTTA-CTCGAG-TAAACTAGCTTAATTACAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTGTAATTAAGCTAGTTTACTCGAGTAAACTAGCTTAATTACAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTGTAATTAAGCTAGTTTACTCGAGTAAACTAGCTTAATTACAGGC-3'
Other clones with same target sequence:
(none)
