Construct: shRNA TRCN0000285870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030245.2-1636s21c1
DNA Barcode:
GCTTTGATCCTTGAGTCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tada1 (27878)
Gene Description:
transcriptional adaptor 1
Transcript:
RefSeq NM_030245.2 (NON-CURRENT)
Match location:
Position 1636 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGATCCTTGAGTCCAAT
Hairpin Sequence:
5'-CCGG-GCTTTGATCCTTGAGTCCAAT-CTCGAG-ATTGGACTCAAGGATCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGATCCTTGAGTCCAATCTCGAGATTGGACTCAAGGATCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGATCCTTGAGTCCAATCTCGAGATTGGACTCAAGGATCAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000192592