Construct: shRNA TRCN0000285941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133732.2-251s21c1
DNA Barcode:
CAGATTTAACAAAGGAGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4931406C07Rik (70984)
Gene Description:
RIKEN cDNA 4931406C07 gene
Transcript:
RefSeq NM_133732.2 (NON-CURRENT)
Match location:
Position 251 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATTTAACAAAGGAGCCAT
Hairpin Sequence:
5'-CCGG-CAGATTTAACAAAGGAGCCAT-CTCGAG-ATGGCTCCTTTGTTAAATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATTTAACAAAGGAGCCATCTCGAGATGGCTCCTTTGTTAAATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATTTAACAAAGGAGCCATCTCGAGATGGCTCCTTTGTTAAATCTG-3'

Other clones with same target sequence:

  1. TRCN0000177094