Construct: shRNA TRCN0000285958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172843.2-3566s21c1
DNA Barcode:
GCTGACTTTGAACTTAGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tor1aip2 (240832)
Gene Description:
torsin A interacting protein 2
Transcript:
RefSeq NM_172843.2 (NON-CURRENT)
Match location:
Position 3566 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTTTGAACTTAGAGTA
Hairpin Sequence:
5'-CCGG-GCTGACTTTGAACTTAGAGTA-CTCGAG-TACTCTAAGTTCAAAGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTTTGAACTTAGAGTACTCGAGTACTCTAAGTTCAAAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTTTGAACTTAGAGTACTCGAGTACTCTAAGTTCAAAGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000179322