Construct: shRNA TRCN0000285968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025509.2-520s21c1
DNA Barcode:
CCCGGATTTGCTCCTGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ostc (66357)
Gene Description:
oligosaccharyltransferase complex subunit (non-catalytic)
Transcript:
RefSeq NM_025509.2 (NON-CURRENT)
Match location:
Position 520 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGATTTGCTCCTGTTAAT
Hairpin Sequence:
5'-CCGG-CCCGGATTTGCTCCTGTTAAT-CTCGAG-ATTAACAGGAGCAAATCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGATTTGCTCCTGTTAATCTCGAGATTAACAGGAGCAAATCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGATTTGCTCCTGTTAATCTCGAGATTAACAGGAGCAAATCCGGG-3'

Other clones with same target sequence:

  1. TRCN0000202196