Construct: shRNA TRCN0000285989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024546.3-2028s21c1
DNA Barcode:
AGCAGTTCACATCGACTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OBI1 (79596)
Gene Description:
ORC ubiquitin ligase 1
Transcript:
RefSeq NM_024546.3 (NON-CURRENT)
Match location:
Position 2028 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTTCACATCGACTATTT
Hairpin Sequence:
5'-CCGG-AGCAGTTCACATCGACTATTT-CTCGAG-AAATAGTCGATGTGAACTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTTCACATCGACTATTTCTCGAGAAATAGTCGATGTGAACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTTCACATCGACTATTTCTCGAGAAATAGTCGATGTGAACTGCT-3'

Other clones with same target sequence:

(none)