Construct: shRNA TRCN0000286024

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006986.3-2119s21c1
DNA Barcode:
CAAGATGAAAGTGCTGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGED1 (9500)
Gene Description:
MAGE family member D1
Transcript:
RefSeq NM_006986.3 (NON-CURRENT)
Match location:
Position 2119 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286024)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGATGAAAGTGCTGAGATT
Hairpin Sequence:
5'-CCGG-CAAGATGAAAGTGCTGAGATT-CTCGAG-AATCTCAGCACTTTCATCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGATGAAAGTGCTGAGATTCTCGAGAATCTCAGCACTTTCATCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGATGAAAGTGCTGAGATTCTCGAGAATCTCAGCACTTTCATCTTG-3'

Other clones with same target sequence:

  1. TRCN0000004238