Construct: shRNA TRCN0000286129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016391.4-337s21c1
DNA Barcode:
CGGGACCTCATTGACTATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOP16 (51491)
Gene Description:
NOP16 nucleolar protein
Transcript:
RefSeq NM_016391.4 (NON-CURRENT)
Match location:
Position 337 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGACCTCATTGACTATGTA
Hairpin Sequence:
5'-CCGG-CGGGACCTCATTGACTATGTA-CTCGAG-TACATAGTCAATGAGGTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGACCTCATTGACTATGTACTCGAGTACATAGTCAATGAGGTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGACCTCATTGACTATGTACTCGAGTACATAGTCAATGAGGTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000072545