Construct: shRNA TRCN0000286209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021177.3-362s21c1
DNA Barcode:
CCCTCACATGTTATCAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSM2 (57819)
Gene Description:
LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
Transcript:
RefSeq NM_021177.3 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCACATGTTATCAGTGAA
Hairpin Sequence:
5'-CCGG-CCCTCACATGTTATCAGTGAA-CTCGAG-TTCACTGATAACATGTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCACATGTTATCAGTGAACTCGAGTTCACTGATAACATGTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCACATGTTATCAGTGAACTCGAGTTCACTGATAACATGTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000074680