Construct: shRNA TRCN0000286235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006185.2-2371s21c1
DNA Barcode:
GCGTTGTATCTCTGAGCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUMA1 (4926)
Gene Description:
nuclear mitotic apparatus protein 1
Transcript:
RefSeq NM_006185.2 (NON-CURRENT)
Match location:
Position 2371 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTGTATCTCTGAGCTGAA
Hairpin Sequence:
5'-CCGG-GCGTTGTATCTCTGAGCTGAA-CTCGAG-TTCAGCTCAGAGATACAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTGTATCTCTGAGCTGAACTCGAGTTCAGCTCAGAGATACAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTGTATCTCTGAGCTGAACTCGAGTTCAGCTCAGAGATACAACGC-3'

Other clones with same target sequence:

  1. TRCN0000074172