Construct: shRNA TRCN0000286311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006452.3-1390s21c1
DNA Barcode:
GCTGCTCAGATATTTGGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAICS (10606)
Gene Description:
phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase
Transcript:
RefSeq NM_006452.3 (CURRENT)
Match location:
Position 1390 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCAGATATTTGGGTTA
Hairpin Sequence:
5'-CCGG-GCTGCTCAGATATTTGGGTTA-CTCGAG-TAACCCAAATATCTGAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCAGATATTTGGGTTACTCGAGTAACCCAAATATCTGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCAGATATTTGGGTTACTCGAGTAACCCAAATATCTGAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000045775