Construct: shRNA TRCN0000286339
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004463.2-2957s21c1
- DNA Barcode:
- GCCCTTCAATTCTATCACCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGD1 (2245)
- Gene Description:
- FYVE, RhoGEF and PH domain containing 1
- Transcript:
- RefSeq NM_004463.2 (NON-CURRENT)
- Match location:
- Position 2957 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000286339)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCCTTCAATTCTATCACCAA
- Hairpin Sequence:
-
5'-CCGG-GCCCTTCAATTCTATCACCAA-CTCGAG-TTGGTGATAGAATTGAAGGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCCTTCAATTCTATCACCAACTCGAGTTGGTGATAGAATTGAAGGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCCTTCAATTCTATCACCAACTCGAGTTGGTGATAGAATTGAAGGGC-3'
Other clones with same target sequence:
-
TRCN0000048168
