Construct: shRNA TRCN0000286359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002035.2-295s21c1
DNA Barcode:
GCATTGCTATCGAGTGCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDSR (2531)
Gene Description:
3-ketodihydrosphingosine reductase
Transcript:
RefSeq NM_002035.2 (NON-CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGCTATCGAGTGCTATA
Hairpin Sequence:
5'-CCGG-GCATTGCTATCGAGTGCTATA-CTCGAG-TATAGCACTCGATAGCAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGCTATCGAGTGCTATACTCGAGTATAGCACTCGATAGCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGCTATCGAGTGCTATACTCGAGTATAGCACTCGATAGCAATGC-3'

Other clones with same target sequence:

  1. TRCN0000064865