Construct: shRNA TRCN0000286363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002078.3-2300s21c1
DNA Barcode:
GCCCACATAGAAGAAATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLGA4 (2803)
Gene Description:
golgin A4
Transcript:
RefSeq NM_002078.3 (NON-CURRENT)
Match location:
Position 2300 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACATAGAAGAAATGAAT
Hairpin Sequence:
5'-CCGG-GCCCACATAGAAGAAATGAAT-CTCGAG-ATTCATTTCTTCTATGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACATAGAAGAAATGAATCTCGAGATTCATTTCTTCTATGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACATAGAAGAAATGAATCTCGAGATTCATTTCTTCTATGTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000061988