Construct: shRNA TRCN0000286497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002858.3-232s21c1
DNA Barcode:
CCATTACAGAACAATGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCD3 (5825)
Gene Description:
ATP binding cassette subfamily D member 3
Transcript:
RefSeq NM_002858.3 (NON-CURRENT)
Match location:
Position 232 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTACAGAACAATGAGAAA
Hairpin Sequence:
5'-CCGG-CCATTACAGAACAATGAGAAA-CTCGAG-TTTCTCATTGTTCTGTAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTACAGAACAATGAGAAACTCGAGTTTCTCATTGTTCTGTAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTACAGAACAATGAGAAACTCGAGTTTCTCATTGTTCTGTAATGG-3'

Other clones with same target sequence:

  1. TRCN0000059848