Construct: shRNA TRCN0000286500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006987.2-631s21c1
DNA Barcode:
CAGGAAGAAAGTCTGCACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPH3AL (9501)
Gene Description:
rabphilin 3A like (without C2 domains)
Transcript:
RefSeq NM_006987.2 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAGAAAGTCTGCACCAA
Hairpin Sequence:
5'-CCGG-CAGGAAGAAAGTCTGCACCAA-CTCGAG-TTGGTGCAGACTTTCTTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAGAAAGTCTGCACCAACTCGAGTTGGTGCAGACTTTCTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAGAAAGTCTGCACCAACTCGAGTTGGTGCAGACTTTCTTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000059864