Construct: shRNA TRCN0000286520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000126.3-239s21c1
DNA Barcode:
GCTTAGTAGCTGGAACCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETFA (2108)
Gene Description:
electron transfer flavoprotein subunit alpha
Transcript:
RefSeq NM_000126.3 (NON-CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGTAGCTGGAACCAAAT
Hairpin Sequence:
5'-CCGG-GCTTAGTAGCTGGAACCAAAT-CTCGAG-ATTTGGTTCCAGCTACTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGTAGCTGGAACCAAATCTCGAGATTTGGTTCCAGCTACTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGTAGCTGGAACCAAATCTCGAGATTTGGTTCCAGCTACTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000064413