Construct: shRNA TRCN0000286530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001128.5-1425s21c1
DNA Barcode:
GCCCTGGTAAATGGGAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP1G1 (164)
Gene Description:
adaptor related protein complex 1 subunit gamma 1
Transcript:
RefSeq NM_001128.5 (NON-CURRENT)
Match location:
Position 1425 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGGTAAATGGGAATAAT
Hairpin Sequence:
5'-CCGG-GCCCTGGTAAATGGGAATAAT-CTCGAG-ATTATTCCCATTTACCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGGTAAATGGGAATAATCTCGAGATTATTCCCATTTACCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGGTAAATGGGAATAATCTCGAGATTATTCCCATTTACCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000065148