Construct: shRNA TRCN0000286588
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002396.3-1539s21c1
- DNA Barcode:
- GCACGGCTGAAGAAGCATATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ME2 (4200)
- Gene Description:
- malic enzyme 2
- Transcript:
- RefSeq NM_002396.3 (NON-CURRENT)
- Match location:
- Position 1539 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000286588)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACGGCTGAAGAAGCATATA
- Hairpin Sequence:
-
5'-CCGG-GCACGGCTGAAGAAGCATATA-CTCGAG-TATATGCTTCTTCAGCCGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACGGCTGAAGAAGCATATACTCGAGTATATGCTTCTTCAGCCGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACGGCTGAAGAAGCATATACTCGAGTATATGCTTCTTCAGCCGTGC-3'
Other clones with same target sequence:
-
TRCN0000064742
