Construct: shRNA TRCN0000286589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198080.2-384s21c1
DNA Barcode:
CATTCCACGATGTGATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MSRB3 (253827)
Gene Description:
methionine sulfoxide reductase B3
Transcript:
RefSeq NM_198080.2 (NON-CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCCACGATGTGATCAATT
Hairpin Sequence:
5'-CCGG-CATTCCACGATGTGATCAATT-CTCGAG-AATTGATCACATCGTGGAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCCACGATGTGATCAATTCTCGAGAATTGATCACATCGTGGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCCACGATGTGATCAATTCTCGAGAATTGATCACATCGTGGAATG-3'

Other clones with same target sequence:

  1. TRCN0000064761