Construct: shRNA TRCN0000286636

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001662.2-388s21c1
DNA Barcode:
GATGCAGTGCTGCTGGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARF5 (381)
Gene Description:
ADP ribosylation factor 5
Transcript:
RefSeq NM_001662.2 (NON-CURRENT)
Match location:
Position 388 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286636)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCAGTGCTGCTGGTATTT
Hairpin Sequence:
5'-CCGG-GATGCAGTGCTGCTGGTATTT-CTCGAG-AAATACCAGCAGCACTGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCAGTGCTGCTGGTATTTCTCGAGAAATACCAGCAGCACTGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCAGTGCTGCTGGTATTTCTCGAGAAATACCAGCAGCACTGCATC-3'

Other clones with same target sequence:

  1. TRCN0000047991