Construct: shRNA TRCN0000286658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004163.3-455s21c1
DNA Barcode:
GCCAACTGCAAGCAAATGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB27B (5874)
Gene Description:
RAB27B, member RAS oncogene family
Transcript:
RefSeq NM_004163.3 (NON-CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACTGCAAGCAAATGCTT
Hairpin Sequence:
5'-CCGG-GCCAACTGCAAGCAAATGCTT-CTCGAG-AAGCATTTGCTTGCAGTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACTGCAAGCAAATGCTTCTCGAGAAGCATTTGCTTGCAGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACTGCAAGCAAATGCTTCTCGAGAAGCATTTGCTTGCAGTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000047691