Construct: shRNA TRCN0000286687

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019027.3-1885s21c1
DNA Barcode:
CGCTTGACTATTTATGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM47 (54502)
Gene Description:
RNA binding motif protein 47
Transcript:
RefSeq NM_019027.3 (NON-CURRENT)
Match location:
Position 1885 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286687)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTGACTATTTATGAAGAA
Hairpin Sequence:
5'-CCGG-CGCTTGACTATTTATGAAGAA-CTCGAG-TTCTTCATAAATAGTCAAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTGACTATTTATGAAGAACTCGAGTTCTTCATAAATAGTCAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTGACTATTTATGAAGAACTCGAGTTCTTCATAAATAGTCAAGCG-3'

Other clones with same target sequence:

  1. TRCN0000074563