Construct: shRNA TRCN0000286693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007363.4-2069s21c1
DNA Barcode:
GCCAGAATTCTACCCTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NONO (4841)
Gene Description:
non-POU domain containing octamer binding
Transcript:
RefSeq NM_007363.4 (NON-CURRENT)
Match location:
Position 2069 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAATTCTACCCTGGAAA
Hairpin Sequence:
5'-CCGG-GCCAGAATTCTACCCTGGAAA-CTCGAG-TTTCCAGGGTAGAATTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAATTCTACCCTGGAAACTCGAGTTTCCAGGGTAGAATTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAATTCTACCCTGGAAACTCGAGTTTCCAGGGTAGAATTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000074558