Construct: shRNA TRCN0000286716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012341.2-1643s21c1
DNA Barcode:
GCGTAGTCTTGGTGTTGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTPBP4 (23560)
Gene Description:
GTP binding protein 4
Transcript:
RefSeq NM_012341.2 (NON-CURRENT)
Match location:
Position 1643 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTAGTCTTGGTGTTGACAT
Hairpin Sequence:
5'-CCGG-GCGTAGTCTTGGTGTTGACAT-CTCGAG-ATGTCAACACCAAGACTACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTAGTCTTGGTGTTGACATCTCGAGATGTCAACACCAAGACTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTAGTCTTGGTGTTGACATCTCGAGATGTCAACACCAAGACTACGC-3'

Other clones with same target sequence:

  1. TRCN0000047976