Construct: shRNA TRCN0000286722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152726.1-619s21c1
DNA Barcode:
GCTGCAGAAGATCATAAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MICU2 (221154)
Gene Description:
mitochondrial calcium uptake 2
Transcript:
RefSeq NM_152726.1 (NON-CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAGAAGATCATAAGTAA
Hairpin Sequence:
5'-CCGG-GCTGCAGAAGATCATAAGTAA-CTCGAG-TTACTTATGATCTTCTGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAGAAGATCATAAGTAACTCGAGTTACTTATGATCTTCTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAGAAGATCATAAGTAACTCGAGTTACTTATGATCTTCTGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000055850