Construct: shRNA TRCN0000286753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138933.1-1245s21c1
DNA Barcode:
CCATGCTGCAAGGAGAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
A1CF (29974)
Gene Description:
APOBEC1 complementation factor
Transcript:
RefSeq NM_138933.1 (NON-CURRENT)
Match location:
Position 1245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCTGCAAGGAGAGTATA
Hairpin Sequence:
5'-CCGG-CCATGCTGCAAGGAGAGTATA-CTCGAG-TATACTCTCCTTGCAGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCTGCAAGGAGAGTATACTCGAGTATACTCTCCTTGCAGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCTGCAAGGAGAGTATACTCGAGTATACTCTCCTTGCAGCATGG-3'

Other clones with same target sequence:

  1. TRCN0000061845