Construct: shRNA TRCN0000286826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014666.2-332s21c1
DNA Barcode:
GCCAAGGCTACATTTATGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLINT1 (9685)
Gene Description:
clathrin interactor 1
Transcript:
RefSeq NM_014666.2 (NON-CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGCTACATTTATGTAT
Hairpin Sequence:
5'-CCGG-GCCAAGGCTACATTTATGTAT-CTCGAG-ATACATAAATGTAGCCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGCTACATTTATGTATCTCGAGATACATAAATGTAGCCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGCTACATTTATGTATCTCGAGATACATAAATGTAGCCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000061907