Construct: shRNA TRCN0000286850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021831.5-1063s21c1
DNA Barcode:
CCTCTTCGTCTTTAAGCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGBL5 (60509)
Gene Description:
ATP/GTP binding protein like 5
Transcript:
RefSeq NM_021831.5 (NON-CURRENT)
Match location:
Position 1063 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTCGTCTTTAAGCTGAT
Hairpin Sequence:
5'-CCGG-CCTCTTCGTCTTTAAGCTGAT-CTCGAG-ATCAGCTTAAAGACGAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTCGTCTTTAAGCTGATCTCGAGATCAGCTTAAAGACGAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTCGTCTTTAAGCTGATCTCGAGATCAGCTTAAAGACGAAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000073892