Construct: shRNA TRCN0000286892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022719.2-256s21c1
DNA Barcode:
GAATGGAGACTTGGAACGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ESS2 (8220)
Gene Description:
ess-2 splicing factor homolog
Transcript:
RefSeq NM_022719.2 (NON-CURRENT)
Match location:
Position 256 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGAGACTTGGAACGGAT
Hairpin Sequence:
5'-CCGG-GAATGGAGACTTGGAACGGAT-CTCGAG-ATCCGTTCCAAGTCTCCATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGAGACTTGGAACGGATCTCGAGATCCGTTCCAAGTCTCCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGAGACTTGGAACGGATCTCGAGATCCGTTCCAAGTCTCCATTC-3'

Other clones with same target sequence:

  1. TRCN0000063892