Construct: shRNA TRCN0000286904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004863.2-1544s21c1
DNA Barcode:
CCTGAAAGAGATGGGCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPTLC2 (9517)
Gene Description:
serine palmitoyltransferase long chain base subunit 2
Transcript:
RefSeq NM_004863.2 (NON-CURRENT)
Match location:
Position 1544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAAGAGATGGGCTTCAT
Hairpin Sequence:
5'-CCGG-CCTGAAAGAGATGGGCTTCAT-CTCGAG-ATGAAGCCCATCTCTTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAAGAGATGGGCTTCATCTCGAGATGAAGCCCATCTCTTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAAGAGATGGGCTTCATCTCGAGATGAAGCCCATCTCTTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000034973