Construct: shRNA TRCN0000286918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002428.2-2527s21c1
DNA Barcode:
CCTGAGCAATGACGCAGCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MMP15 (4324)
Gene Description:
matrix metallopeptidase 15
Transcript:
RefSeq NM_002428.2 (NON-CURRENT)
Match location:
Position 2527 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGCAATGACGCAGCCTA
Hairpin Sequence:
5'-CCGG-CCTGAGCAATGACGCAGCCTA-CTCGAG-TAGGCTGCGTCATTGCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGCAATGACGCAGCCTACTCGAGTAGGCTGCGTCATTGCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGCAATGACGCAGCCTACTCGAGTAGGCTGCGTCATTGCTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000051711