Construct: shRNA TRCN0000286920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012419.4-469s21c1
DNA Barcode:
GAATACAGTGAAGAGAACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS17 (26575)
Gene Description:
regulator of G protein signaling 17
Transcript:
RefSeq NM_012419.4 (NON-CURRENT)
Match location:
Position 469 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATACAGTGAAGAGAACCTA
Hairpin Sequence:
5'-CCGG-GAATACAGTGAAGAGAACCTA-CTCGAG-TAGGTTCTCTTCACTGTATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATACAGTGAAGAGAACCTACTCGAGTAGGTTCTCTTCACTGTATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATACAGTGAAGAGAACCTACTCGAGTAGGTTCTCTTCACTGTATTC-3'

Other clones with same target sequence:

  1. TRCN0000036808