Construct: shRNA TRCN0000286933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004731.3-830s21c1
DNA Barcode:
CGAAGAAATCAACTTGGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC16A7 (9194)
Gene Description:
solute carrier family 16 member 7
Transcript:
RefSeq NM_004731.3 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGAAATCAACTTGGGAAA
Hairpin Sequence:
5'-CCGG-CGAAGAAATCAACTTGGGAAA-CTCGAG-TTTCCCAAGTTGATTTCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGAAATCAACTTGGGAAACTCGAGTTTCCCAAGTTGATTTCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGAAATCAACTTGGGAAACTCGAGTTTCCCAAGTTGATTTCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000038505