Construct: shRNA TRCN0000286943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016337.2-357s21c1
DNA Barcode:
CGGCTTAAACTTTGCAAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EVL (51466)
Gene Description:
Enah/Vasp-like
Transcript:
RefSeq NM_016337.2 (NON-CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTTAAACTTTGCAAGTAA
Hairpin Sequence:
5'-CCGG-CGGCTTAAACTTTGCAAGTAA-CTCGAG-TTACTTGCAAAGTTTAAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTTAAACTTTGCAAGTAACTCGAGTTACTTGCAAAGTTTAAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTTAAACTTTGCAAGTAACTCGAGTTACTTGCAAAGTTTAAGCCG-3'

Other clones with same target sequence:

  1. TRCN0000063871