Construct: shRNA TRCN0000286969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000963.2-1230s21c1
DNA Barcode:
GCTGAATTTAACACCCTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTGS2 (5743)
Gene Description:
prostaglandin-endoperoxide synthase 2
Transcript:
RefSeq NM_000963.2 (NON-CURRENT)
Match location:
Position 1230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000286969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATTTAACACCCTCTAT
Hairpin Sequence:
5'-CCGG-GCTGAATTTAACACCCTCTAT-CTCGAG-ATAGAGGGTGTTAAATTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATTTAACACCCTCTATCTCGAGATAGAGGGTGTTAAATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATTTAACACCCTCTATCTCGAGATAGAGGGTGTTAAATTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000045533