Construct: shRNA TRCN0000287054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014396.3-2134s21c1
DNA Barcode:
CCATTGACAAACCACCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS41 (27072)
Gene Description:
VPS41 subunit of HOPS complex
Transcript:
RefSeq NM_014396.3 (NON-CURRENT)
Match location:
Position 2134 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGACAAACCACCATTTA
Hairpin Sequence:
5'-CCGG-CCATTGACAAACCACCATTTA-CTCGAG-TAAATGGTGGTTTGTCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGACAAACCACCATTTACTCGAGTAAATGGTGGTTTGTCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGACAAACCACCATTTACTCGAGTAAATGGTGGTTTGTCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000034324