Construct: shRNA TRCN0000287175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011550.3-669s21c1
DNA Barcode:
GAAAGTGAACTATGAGCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mlx (21428)
Gene Description:
MAX-like protein X
Transcript:
RefSeq NM_011550.3 (CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGTGAACTATGAGCAAAT
Hairpin Sequence:
5'-CCGG-GAAAGTGAACTATGAGCAAAT-CTCGAG-ATTTGCTCATAGTTCACTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGTGAACTATGAGCAAATCTCGAGATTTGCTCATAGTTCACTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGTGAACTATGAGCAAATCTCGAGATTTGCTCATAGTTCACTTTC-3'

Other clones with same target sequence:

  1. TRCN0000054362