Construct: shRNA TRCN0000287188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024190.1-904s21c1
DNA Barcode:
GCTCTACATAAATCTAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp1b (67064)
Gene Description:
charged multivesicular body protein 1B
Transcript:
RefSeq NM_024190.1 (NON-CURRENT)
Match location:
Position 904 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTACATAAATCTAGGAAA
Hairpin Sequence:
5'-CCGG-GCTCTACATAAATCTAGGAAA-CTCGAG-TTTCCTAGATTTATGTAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTACATAAATCTAGGAAACTCGAGTTTCCTAGATTTATGTAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTACATAAATCTAGGAAACTCGAGTTTCCTAGATTTATGTAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000100770