Construct: shRNA TRCN0000287344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130884.3-248s21c1
DNA Barcode:
CCTGTGGAATTTAAGGAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Idh3b (170718)
Gene Description:
isocitrate dehydrogenase 3 (NAD+) beta
Transcript:
RefSeq NM_130884.3 (NON-CURRENT)
Match location:
Position 248 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGGAATTTAAGGAGCAT
Hairpin Sequence:
5'-CCGG-CCTGTGGAATTTAAGGAGCAT-CTCGAG-ATGCTCCTTAAATTCCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGGAATTTAAGGAGCATCTCGAGATGCTCCTTAAATTCCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGGAATTTAAGGAGCATCTCGAGATGCTCCTTAAATTCCACAGG-3'

Other clones with same target sequence:

  1. TRCN0000041395