Construct: shRNA TRCN0000287483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029993.1-1376s21c1
DNA Barcode:
GACTATGAGATAGAACGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc3h18 (76014)
Gene Description:
zinc finger CCCH-type containing 18
Transcript:
RefSeq NM_001029993.1 (CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTATGAGATAGAACGGTTT
Hairpin Sequence:
5'-CCGG-GACTATGAGATAGAACGGTTT-CTCGAG-AAACCGTTCTATCTCATAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTATGAGATAGAACGGTTTCTCGAGAAACCGTTCTATCTCATAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTATGAGATAGAACGGTTTCTCGAGAAACCGTTCTATCTCATAGTC-3'

Other clones with same target sequence:

  1. TRCN0000123496