Construct: shRNA TRCN0000287513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010515.2-2568s21c1
DNA Barcode:
CGGAGGAAATACTACCTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igf2r (16004)
Gene Description:
insulin-like growth factor 2 receptor
Transcript:
RefSeq NM_010515.2 (CURRENT)
Match location:
Position 2568 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGAAATACTACCTCAAT
Hairpin Sequence:
5'-CCGG-CGGAGGAAATACTACCTCAAT-CTCGAG-ATTGAGGTAGTATTTCCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGAAATACTACCTCAATCTCGAGATTGAGGTAGTATTTCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGAAATACTACCTCAATCTCGAGATTGAGGTAGTATTTCCTCCG-3'

Other clones with same target sequence:

  1. TRCN0000119863