Construct: shRNA TRCN0000287556

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172601.2-154s21c1
DNA Barcode:
CCTCCTTCAGTTTACCGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab2b (76338)
Gene Description:
RAB2B, member RAS oncogene family
Transcript:
RefSeq NM_172601.2 (NON-CURRENT)
Match location:
Position 154 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287556)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTTCAGTTTACCGACAA
Hairpin Sequence:
5'-CCGG-CCTCCTTCAGTTTACCGACAA-CTCGAG-TTGTCGGTAAACTGAAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTTCAGTTTACCGACAACTCGAGTTGTCGGTAAACTGAAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTTCAGTTTACCGACAACTCGAGTTGTCGGTAAACTGAAGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000100407