Construct: shRNA TRCN0000287632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148930.2-485s21c1
DNA Barcode:
CGAGAGCGATATTCGAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm5 (83486)
Gene Description:
RNA binding motif protein 5
Transcript:
RefSeq NM_148930.2 (NON-CURRENT)
Match location:
Position 485 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAGCGATATTCGAGAAAT
Hairpin Sequence:
5'-CCGG-CGAGAGCGATATTCGAGAAAT-CTCGAG-ATTTCTCGAATATCGCTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAGCGATATTCGAGAAATCTCGAGATTTCTCGAATATCGCTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAGCGATATTCGAGAAATCTCGAGATTTCTCGAATATCGCTCTCG-3'

Other clones with same target sequence:

  1. TRCN0000054663