Construct: shRNA TRCN0000287712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010072.3-644s21c1
DNA Barcode:
CTATGTCTTTCAGATGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpm1 (13480)
Gene Description:
dolichol-phosphate (beta-D) mannosyltransferase 1
Transcript:
RefSeq NM_010072.3 (NON-CURRENT)
Match location:
Position 644 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGTCTTTCAGATGGAAAT
Hairpin Sequence:
5'-CCGG-CTATGTCTTTCAGATGGAAAT-CTCGAG-ATTTCCATCTGAAAGACATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGTCTTTCAGATGGAAATCTCGAGATTTCCATCTGAAAGACATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGTCTTTCAGATGGAAATCTCGAGATTTCCATCTGAAAGACATAG-3'

Other clones with same target sequence:

  1. TRCN0000111998